FastQCFastQC Report
Mon 22 May 2023
EGAF00007850441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007850441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4405025
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC195300.4433573021719514Illumina Single End PCR Primer 1 (96% over 31bp)
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG100860.2289657833950999No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC90120.2045845369776562No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG86840.1971384952412302No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG67270.15271195963700546No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT52790.1198404095322955No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT52450.11906856374254403No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA49060.11137280719178665No Hit
CCTCGATGTTGGATCAGGACATCCCAATGGTGCAGCCGCTATTAAAGGTT48590.11030584389418903No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA47320.10742277285599969No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA46830.10631040686488727No Hit
CGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGA46260.10501643009971566No Hit
CATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGG45860.10410837622941982No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)