FastQCFastQC Report
Wed 8 May 2024
EGAF00007850880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007850880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1980303
Total Bases148.5 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC66480.33570620253567257No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA51950.26233359238459975No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT49550.2502142348923372No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG38750.1956771261771557No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT38170.19274828144985892No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG34830.17588217560646022No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA34230.17285233623339458No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA33490.16911553433994697No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA32780.16553022441515264No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG31100.15704667417056883No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG28920.1460382577817637No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG27860.1406855415560144No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT27490.1388171406092906No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT25520.12886916800105844No Hit
CCCGGCCGTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATA25480.1286671787095207No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT23570.11902219003859511No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT23530.1188202007470574No Hit
GTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTT23290.11760826499783114No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC23230.11730528106052458No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA23090.1165983185401426No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT22950.11589135601976061No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG22830.1152853881451475No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT22330.11276052200092614No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC22120.11170007822035315No Hit
CCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGGTC22040.11129609963727774No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG21840.11028615317958919No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT21810.1101346612109359No Hit
CCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGGT21500.10856924420151864No Hit
GTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAA21210.10710482183787028No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA21070.10639785931748828No Hit
CCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCG20660.10432746907922677No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC20600.1040244851419202No Hit
CTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTT20500.10351951191307593No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA20430.10316603065288493No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT20360.10281254939269395No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG19840.10018668860270373No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)