FastQCFastQC Report
Wed 8 May 2024
EGAF00007851356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007851356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3361077
Total Bases339.4 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG447261.3307044140910784TruSeq Adapter, Index 19 (97% over 37bp)
CGGCCTTCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACT293080.8719824032594313No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC278730.8292877550856467No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC201070.5982308646900979No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG192000.5712454668548206No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC93680.27872018403624793No Hit
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT81180.24152972395455385No Hit
CGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCC77500.23058085250650312No Hit
GGCCTTCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACTC74300.22106009472558943No Hit
TTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTT71220.21189636536146No Hit
GCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA69690.20734425304746068No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA69480.20671945331808822No Hit
GGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGT69410.20651118674163074No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC68750.2045475304493173No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG68610.2041309972964023No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT63610.18925481326372467No Hit
CCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGT58100.17286125845971395No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA58100.17286125845971395No Hit
TCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC56870.16920171718767524No Hit
TCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCA55380.16476861434593731No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC54490.1621206535881207No Hit
CCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAAC54280.16149585385874823No Hit
CGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTC53690.1597404641428923No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG51320.15268915291140311No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA49740.14798827875707699No Hit
CTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT48640.14471551826988788No Hit
GGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCAT46810.1392708349139279No Hit
GTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTG46310.1377832165106601No Hit
GCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGG45110.1342129323428175No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG43550.12957156292462207No Hit
TCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG42760.127221125847459No Hit
TGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTT42470.12635830717356372No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG41830.12445415561738098No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC40770.12130040460245334No Hit
GAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAG40220.11966402435885878No Hit
TCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGC39730.11820615832365637No Hit
CGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAACT38620.11490364546840194No Hit
CTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGG36050.10725728687560564No Hit
TTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCATCGGGATGT35570.10582917320846859No Hit
GGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCACGCCCT34990.104103535860678No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT34770.10344898376324017No Hit
TCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCC34240.10187210825577636No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC33840.10068201353316214No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)