FastQCFastQC Report
Wed 31 May 2023
EGAF00007852454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007852454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3260010
Sequences flagged as poor quality0
Sequence length75
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTT777942.3863116984303727No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGT588431.8049944632071682No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCC537391.6484305262867291No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTC535631.6430317698411967No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATC498211.528246845868571No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCG435881.3370511133401433No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTT302010.9264082012018369No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA290790.891991128861568No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGG242850.7449363652258736No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGG241360.7403658270986899No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG222650.6829733651123769No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT216640.6645378388409852No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGT202300.6205502437109088No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCC185010.5675135965840595No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACC184480.5658878346998936No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCG174890.5364707470222484No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGT167170.5127898380679814No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTT166500.5107346296483752No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCGCGGTGGAAATG162660.4989555246763046No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA162300.497851233585173No Hit
GGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCC158900.48742181772448545No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACC151640.46515194738666443No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCC150130.46052005975441795No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT137960.4231888859236628No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG135930.4169619111597817No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGG132940.40779016015288294No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCC132120.4052748304453054No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCG129090.39598038042828093No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAG126860.3891399106137711No Hit
CCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCGCGGTGGAAATGC125760.3857656878353134No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCAC123210.37794362593979774No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT122740.37650191257082033No Hit
GTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTC121180.3717166511759166No Hit
GTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT117280.3597534976886574No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGC112480.3450296164735691No Hit
GGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCC104330.32002969316045043No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTC100110.3070849475921853No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG97170.2980665703479437No Hit
TATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCA95060.29159419756381116No Hit
GCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGT92770.28456967923411275No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGA90490.2775758356569459No Hit
CGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG88980.2729439480246993No Hit
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGC87340.2679132886095441No Hit
GGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATTC85200.26134889156781727No Hit
TGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG83730.25683970294569647No Hit
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGG79160.24282134103883116No Hit
GGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATTCCCC76380.23429375983509254No Hit
TCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT75850.23266799795092652No Hit
ACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCA67680.20760672513274497No Hit
TGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTC66400.20368035680872146No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCG64450.19769878006509184No Hit
GGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGA63940.19613436768598871No Hit
GTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT63380.1944165815442284No Hit
CTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGG62390.19137978104361641No Hit
CCGGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGT62350.1912570820334907No Hit
GGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAG60540.18570495182530114No Hit
CGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTC59090.18125711270824324No Hit
CCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCGCGGTGGAAATGCG57460.1762571280456195No Hit
GTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGC57040.17496878843929925No Hit
GGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATT56210.17242278397919025No Hit
CGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTG54690.16776022159441228No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT50430.15469277701602144No Hit
GGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCC49900.15306701513185542No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGG48430.14855782650973465No Hit
GGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATTCCCCGC48300.148159054726826No Hit
GACGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC48130.14763758393379162No Hit
GCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATTCCCCGCGGGG47830.14671734135784859No Hit
CGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGC47580.14595047254456275No Hit
CCGGCCTTCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCACGTGTTAGACTCCTTGGT44730.13720816807310407No Hit
GTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTG44710.1371468185680412No Hit
ACGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG43930.13475418787058935No Hit
GGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCC43860.1345394646028693No Hit
GGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATTCCCCGCGGG43030.1319934601427603No Hit
TGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCAC42180.12938610617758842No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCAT42140.12926340716746268No Hit
CGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCGCGGTGGAAATGCGC41910.1285578878592397No Hit
CCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATTCCCCGCGGGGG41430.12708549973773087No Hit
TGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCA41380.1269321259750737No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTCTCGTGCCG39860.12226956359029574No Hit
CTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCA39150.12009165616056391No Hit
CCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCAC38020.11662540912451189No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGG37150.11395670565427712No Hit
GCCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCA36930.1132818610985856No Hit
GGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATTCCCCG34960.10723893484989311No Hit
CGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG34240.10503035266762985No Hit
CACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCT34150.10475427989484695No Hit
GGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTT33790.10364998880371533No Hit
CGGGTGGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTG32950.10107330959107487No Hit
CCGAGAACCGCCTCGCGAGCCCCGGGGCCCCGCCACCGGGGGCCCCGGAGCGACCGCAGCCACGAACCCGACACG32650.10015306701513185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)