FastQCFastQC Report
Thu 9 May 2024
EGAF00007852559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007852559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4052371
Total Bases409.2 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC285580.704723234866699Illumina Single End Sequencing Primer (100% over 31bp)
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT60480.149245959957763No Hit
TCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGT55030.13579704326183364No Hit
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC46160.11390862287781647No Hit
CCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAG43910.10835631781986398No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)