FastQCFastQC Report
Mon 22 May 2023
EGAF00007852561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007852561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3771375
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC277420.7355937821086473Illumina Single End PCR Primer 1 (96% over 31bp)
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT56720.1503960757018329No Hit
TCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGT52000.1378807464121176No Hit
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC44340.11756985184448643No Hit
CCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAG41280.10945610022869642No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)