FastQCFastQC Report
Wed 8 May 2024
EGAF00007852575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007852575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3893755
Total Bases393.2 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC224800.577334732154437Illumina Single End Sequencing Primer (100% over 31bp)
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT58390.14995807389011379No Hit
TCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGT51740.13287944413554525No Hit
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC44730.1148762569807294No Hit
CCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAG39480.10139312822712267No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)