FastQCFastQC Report
Mon 22 May 2023
EGAF00007852589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007852589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3895247
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC223230.573083041973975Illumina Single End PCR Primer 1 (96% over 31bp)
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT56780.14576739292784258No Hit
TCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGT50260.1290290448847018No Hit
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC43830.11252174765810742No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)