FastQCFastQC Report
Wed 8 May 2024
EGAF00007852614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007852614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2969447
Total Bases299.9 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG173750.5851257826794012TruSeq Adapter, Index 7 (97% over 34bp)
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA47820.16104008591498686No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC43180.14541428084084343No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC42490.14309061586214536No Hit
TGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG41440.13955460393803965No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG40780.13733196787145888No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC39240.13214581704943715No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)