FastQCFastQC Report
Mon 22 May 2023
EGAF00007852979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007852979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4104808
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCTAAACCATTTAC148210.36106439083143477No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG129520.3155324195431309No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC106170.25864790752697814Illumina Single End PCR Primer 1 (96% over 31bp)
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT84040.20473551990738667No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG76330.1859526681881345No Hit
CTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGT73510.1790826757305092No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT71360.17384491552345444No Hit
CCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTT69470.1692405588763226No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCTAAACCATTTA66840.16283343825094865No Hit
GGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATA63350.15433121354275278No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG63170.1538927033858831No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG58790.14322228956872038No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCTAAACCATTTACCCAAA58730.14307611951643048No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT58710.1430273961656672No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA57340.13968984663838113No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT57310.13961676161223618No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG56250.1370344240217813No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG56170.13683953061872808No Hit
TTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTT56060.13657155218952993No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA55780.13588942527884373No Hit
CAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTG52010.1267050736599617No Hit
GAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTC51730.1260229467492755No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT50500.12302646067733254No Hit
CTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAG50210.12231997209126468No Hit
CTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGAC50040.12190582360977664No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT48360.11781306214565944No Hit
CCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAA47870.11661934005195859No Hit
CTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCTAAAC47660.11610774486894393No Hit
GCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAA44950.10950573084051676No Hit
CAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC44810.10916466738517368No Hit
CTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT44660.10879924225444892No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG44160.10758115848536642No Hit
AATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGAAGAC43610.10624126633937568No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT43290.10546169272716288No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCA42990.10473084246571338No Hit
CTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGAC42990.10473084246571338No Hit
TGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAA42940.10460903408880512No Hit
CCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC42600.10378073712582904No Hit
CATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGG42430.10336658864434098No Hit
CTTAGCTAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAAC42380.10324478026743274No Hit
GTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGA41830.10190488812144198No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT41590.10132020791228237No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA41550.10122276121075578No Hit
CGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCC41500.10110095283384751No Hit
AATAACTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCT41070.10005340079243658No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT41050.10000467744167328No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)