FastQCFastQC Report
Wed 31 May 2023
EGAF00007853557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007853557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3500518
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA298530.8528166402800957No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG182120.5202658577959034No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG132040.37720131706221766No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG127170.36328909035748425No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG112080.3201811846132487No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG110930.31689595654128905No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG109350.3123823388424228No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC108700.3105254708017499Illumina Single End PCR Primer 1 (96% over 31bp)
CGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGG103070.2944421368494605No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT101260.28927147353620236No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCTAAACCATTTAC97980.27990143173096094No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG92980.265617831418093No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG91480.26133275132423256No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA89690.25621922241222583No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA79080.22590942254832No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT74690.21336842147362192No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG72980.20848343016662105No Hit
CGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGG71200.20339846845524004No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT63350.18097321596403732No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC63310.1808589471615344No Hit
GGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAA59390.1696606045162459No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA57690.16480418040987077No Hit
CGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGA57130.16320441717482956No Hit
CATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGG56350.16097617552602214No Hit
CGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAA55990.15994775630349564No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT54630.15606261701839555No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG54500.155691243410261No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG54220.1548913617927404No Hit
CGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGG53800.1536915393664595No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG53490.15280595614706166No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCC52560.15014920648886823No Hit
CCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCC52490.14994923608448807No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT51790.14794953204068656No Hit
GCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGT51540.14723535202504315No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAG50780.14506424477748722No Hit
GCAGCCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGA50710.14486427437310706No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCA50640.14466430396872693No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT50370.14389298955183202No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT49730.14206468871178496No Hit
GTAAACTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAG49110.1402935222729893No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT48750.1392651030504628No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAG47910.136865458197901No Hit
CGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAA47020.1343229773422105No Hit
CGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAA46860.13386590213219873No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC43860.12529574194447793No Hit
CCGGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGA42910.12258185788503301No Hit
CTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCT42620.12175340906688667No Hit
CCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAGAG41630.11892525620493882No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG41360.11815394178804393No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT41270.11789683698241232No Hit
CCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAG41020.11718265696676892No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG41010.11715408976614317No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT40650.11612567054361668No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCTAAACCATTTA40630.11606853614236522No Hit
GGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATA39780.11364032408917767No Hit
CCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTT39030.11149778404224746No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCTAAACCATTTACCCAAA38680.11049793202034672No Hit
CGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAG38550.11012655841221212No Hit
GAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTC38240.10924097519281431No Hit
TGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGA38090.10881246718342828No Hit
CCCAAAGCGGGTGGTAAACTCCATCTAAGGCTAAATACCGGCACGAGACC37470.10704130074463264No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT37270.10646995673211793No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAA37200.10626998632773778No Hit
CGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAACTTTG37140.10609858312398336No Hit
GTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCC36930.1054986719108429No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG36880.10535583590771423No Hit
GATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG36570.1044702526883164No Hit
CCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCA36510.10429884948456199No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG36100.10312759425890683No Hit
GCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACC36030.10292762385452667No Hit
CACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCA35740.10209917503638034No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)