FastQCFastQC Report
Wed 8 May 2024
EGAF00007854589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007854589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1873061
Total Bases140.4 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG267761.4295316596736571No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA139100.7426346499126296No Hit
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT124310.6636729930311933No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC83140.4438723565329693No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA79180.4227304930271892No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA74510.39779804288274645No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC72460.3868533913204108No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT68390.365124253828359No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT58410.3118424867102566No Hit
TGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAG58110.31024083038406114No Hit
GGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCT55560.29662675161139973No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT52440.27996952581896695No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG51040.2724951296300548No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA50950.2720146327321961No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG48280.25775989142905653No Hit
GAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCG47810.2552506298513503No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG46820.24996516397490526No Hit
GCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGG46420.24782962220664465No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA46120.24622796588044918No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT43620.2328808298288203No Hit
GTTACAGCCCCCCCGGCAGCAGCACTCGCCGAATCCCGGGGCCGAGGGAG42190.2252462680072886No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT41330.22065485320552827No Hit
CACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCA39760.21227285176510535No Hit
GGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGG39360.21013730999684474No Hit
GTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCC39000.20821532240541019No Hit
GATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAG38130.20357051905944334No Hit
CGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG36930.1971638937546615No Hit
GTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTT36540.1950817405306074No Hit
CAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGT33610.17943889707809837No Hit
GCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACC33230.1774101323982508No Hit
GGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGG30650.1636358879929698No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG29820.15920463882382901No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA29660.15835042211652478No Hit
GGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGG28940.15450644693365567No Hit
CGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGA28830.153919172947384No Hit
CATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGG28110.15007519776451486No Hit
GTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGC27720.14799304454046078No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG27690.14783287890784122No Hit
CTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGG26790.14302790992925485No Hit
CTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCT26680.14244063594298317No Hit
GACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCT26600.14201352758933106No Hit
GCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGAC25320.13517979393089707No Hit
TGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCG25220.13464590848883193No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT25010.13352474906049508No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG24870.13277730944160387No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCC22810.1217792693350617No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC22090.11793529415219257No Hit
CCGGCCGCCGCCGGGCGCATTTCCACCGCGGCGGTGCGCCGCGACCGGCT22080.11788190560798609No Hit
GTTGTTGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGA21610.11537264403027984No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT21380.11414470751353No Hit
GGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA21230.11334387935043226No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC21090.11259643973154106No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC21080.11254305118733453No Hit
GATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG20910.11163544593582377No Hit
TGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGA20110.10736436239930253No Hit
CGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTC19990.10672369986882435No Hit
CCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTA19610.10469493518897677No Hit
CGGTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCG19110.102025507978651No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG19040.10165178816920538No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)