FastQCFastQC Report
Fri 10 May 2024
EGAF00007855829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007855829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6605324
Total Bases667.1 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACTCGTGTGTGTAGATCTC223280.3380303524853588Illumina Single End PCR Primer 1 (96% over 32bp)
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC129690.19634161776167225No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG113510.1718462258626526No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA77180.11684513886071296No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)