FastQCFastQC Report
Wed 8 May 2024
EGAF00007855837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007855837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6698720
Total Bases676.5 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACTCGTGTGTGTAGATCTC220230.3287643012396398Illumina Single End PCR Primer 1 (96% over 32bp)
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC132050.19712721236295888No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG116610.17407803281821005No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA77900.1162908734803067No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)