FastQCFastQC Report
Wed 8 May 2024
EGAF00007856459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007856459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5578000
Total Bases418.3 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC129160.23155252778773752No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT72130.12931158121190392No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT68180.12223019003226962No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG62220.11154535675869488No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA59030.10582646109716745No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG56710.10166726425242022No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)