FastQCFastQC Report
Thu 9 May 2024
EGAF00007856486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007856486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5227443
Total Bases392 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT136570.2612558377011476No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA130060.24880233031713594No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG109000.20851494698268352No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG104530.19996392117522852No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG103330.19766834377725398No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA86830.16610415455510466No Hit
GCACGGTCAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGT86040.16459289943477146No Hit
GCTACCTTTGCACGGTCAGGGTACCGCGGCCGTTAAACATGTGTCACTGG82440.15770616724084796No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT74950.14337793831515716No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATC74860.14320577001030904No Hit
GATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATT66310.12684978104974076No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC65360.12503244894301094No Hit
GGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTAT64830.12401856892557221No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC64220.12285165041493519No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCAC64080.12258383305183815No Hit
GCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCA62000.11860483222868236No Hit
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT60030.11483625933367424No Hit
CTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGC59610.11403280724438314No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA58290.1115076721066112No Hit
GTCAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC57060.10915470527368734No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG55720.10659131051261582No Hit
GTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTG54760.10475484859423623No Hit
GGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT54380.1040279157515443No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG54060.1034157617787511No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG54010.10332011272050215No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)