FastQCFastQC Report
Mon 22 May 2023
EGAF00007856842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007856842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3437231
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG335770.976861898429288TruSeq Adapter, Index 7 (97% over 35bp)
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC150350.43741604797582706No Hit
TGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG124260.36151192631510654No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC106640.31024973299728764No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG105270.3062639665474913No Hit
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT60410.17575193520598412No Hit
GGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGT51060.14854980651576807No Hit
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT50630.14729879952787578No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC49020.14261479661972093No Hit
ACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAG42590.12390787817286647No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG40500.11782740234799464No Hit
CGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCC40130.11675095447469198No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA38560.11218332430959688No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT37630.10947765803345774No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC37450.10895398068968888No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA36480.10613194167049No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC36260.10549189158366137No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)