FastQCFastQC Report
Mon 22 May 2023
EGAF00007857941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007857941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3593524
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC238570.6638887064619576Illumina Single End PCR Primer 1 (96% over 31bp)
TAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAATG120160.33437928896537217No Hit
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC89050.24780688816882812No Hit
CTCGATGTTGGATCAGGACATCCCAATGGTGCAGCCGCTATTAAAGGTTC53690.14940765666237374No Hit
GGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGG52530.14617962757449235No Hit
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGC44760.1245573982530797No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT44560.12400084151378982No Hit
CCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAAT37650.10477180617132374No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)