FastQCFastQC Report
Wed 8 May 2024
EGAF00007858019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007858019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4589332
Total Bases463.5 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC250300.5453952775698075Illumina Single End Sequencing Primer (100% over 31bp)
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG94590.20610842710878183No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC84120.183294649417388No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG70370.1533338620958344No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT46730.10182309756626891No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)