FastQCFastQC Report
Wed 8 May 2024
EGAF00007858037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007858037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3332535
Total Bases336.5 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC306290.9190901220842392Illumina Single End PCR Primer 1 (96% over 33bp)
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC93350.28011708804258617No Hit
TAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAATG70790.2124208747995145No Hit
CTCGATGTTGGATCAGGACATCCCAATGGTGCAGCCGCTATTAAAGGTTC52680.15807785964738555No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT43520.1305912766107483No Hit
GGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGG39560.11870843066914526No Hit
CCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAAT38240.11474748202194426No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)