FastQCFastQC Report
Wed 8 May 2024
EGAF00007858045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007858045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3660815
Total Bases369.7 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC326180.8910037792130987Illumina Single End PCR Primer 1 (96% over 33bp)
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC102450.27985571518910407No Hit
TAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAATG75520.20629286101592129No Hit
CTCGATGTTGGATCAGGACATCCCAATGGTGCAGCCGCTATTAAAGGTTC59500.16253211375062657No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT47560.1299164257139462No Hit
GGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGG42700.11664069339750849No Hit
CCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAAT40190.10978429666617952No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)