FastQCFastQC Report
Mon 22 May 2023
EGAF00007858073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007858073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3702965
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG94600.2554709536817118No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC89290.24113109359661786Illumina Single End PCR Primer 1 (96% over 33bp)
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC71250.19241337684801235No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG49980.1349729203489636No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT46300.12503493821842765No Hit
CCTCGATGTTGGATCAGGACATCCCAATGGTGCAGCCGCTATTAAAGGTT42240.11407075141136899No Hit
GGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATA42190.1139357244802476No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT39710.10723838869662553No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG38180.10310656460431032No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)