FastQCFastQC Report
Wed 8 May 2024
EGAF00007858239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007858239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3318899
Total Bases335.2 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC380671.1469767534354014Illumina Single End PCR Primer 1 (96% over 32bp)
CCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTACC66330.19985543398578867No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57820.17421440061900045No Hit
CTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTC42260.12733138308818678No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT38700.1166049343472037No Hit
TAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAATG33260.10021395649581381No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)