FastQCFastQC Report
Thu 9 May 2024
EGAF00007858243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007858243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3350041
Total Bases338.3 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC404821.208403121036429Illumina Single End PCR Primer 1 (96% over 32bp)
CCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATTTACC71090.21220635807143853No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60660.18107241075556987No Hit
CTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTC44390.1325058409732896No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT39890.11907316955225324No Hit
TAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAATG34780.10381962489414309No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)