FastQCFastQC Report
Wed 31 May 2023
EGAF00007858445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007858445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3817745
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC524511.3738738443767198Illumina Single End PCR Primer 1 (96% over 31bp)
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC152020.39819317424291045No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG123990.32477286984856246No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT90090.23597699689214446No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT83620.21902981995916437No Hit
CTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGT76480.2003276803453347No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG73630.192862540583512No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG66280.17361033803986384No Hit
GAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTC63120.16533320061973758No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA63100.16528081367404057No Hit
GGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATA62050.16253049902494798No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT60670.15891579977185485No Hit
CTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAAC59430.15566780913864076No Hit
TTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTT58850.15414858771342768No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA58220.15249839892397213No Hit
CCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTT57570.15079582318881957No Hit
CTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAG56680.148464604105303No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG56250.14733828477281746No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT54770.14346165079123932No Hit
GTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCAAGACGAGA53360.13976837111960072No Hit
CCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAA53240.1394540494454187No Hit
AATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCAAGACGAGAAGAC51170.13403200056577902No Hit
CAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTG49910.1307316229868679No Hit
CTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCTAAAC49670.1301029796385039No Hit
GGCGGGCATAACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTAT49020.12840040390335133No Hit
CAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC47680.12489047854165221No Hit
GCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAAC47570.12460235034031869No Hit
CCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAA47470.12434041561183368No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT46710.12234971167534762No Hit
CTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT46570.12198300305546861No Hit
TGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAACT45340.118761205895103No Hit
CACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGG44440.11640379333873793No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC42520.11137464655182575No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT41770.10941013608818817No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA41480.10865052537558167No Hit
CTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGAC40840.1069741431132776No Hit
CGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCC40270.10548111516091305No Hit
GCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAA40020.10482627833970054No Hit
AATAACTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCT39960.10466911750260952No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG39440.10330705691448748No Hit
CTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTC39290.10291415482175997No Hit
GTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCC39280.10288796134891147No Hit
TCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTT39170.10259983314757795No Hit
GCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAAC38970.10207596369060792No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC38840.10173544854357743No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT38810.10165686812503193Illumina Single End PCR Primer 1 (96% over 32bp)
TGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAA38740.10147351381509243No Hit
CCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCT38510.10087106393957689No Hit
GGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAA38350.10045196837400087No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA38350.10045196837400087No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)