FastQCFastQC Report
Wed 31 May 2023
EGAF00007858453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007858453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3804165
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC474001.2460027364743644Illumina Single End PCR Primer 1 (96% over 31bp)
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC157720.4145982101196977No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG123990.325932234800541No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT89400.2350055794109877No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT88310.23214029885664794No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG79130.20800885345404313No Hit
CTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGT76670.20154225697360653No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG66210.17404607844296976No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA65740.17281059049752048No Hit
GGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATA65490.17215341605845172No Hit
GAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTC62410.16405702696912464No Hit
CCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTT61140.16071858081865534No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT60740.15966710171614532No Hit
TTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTT60430.1588522054117001No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA59750.15706469093743305No Hit
CTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAAC58760.15446228015872077No Hit
CTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAG55550.14602416036107793No Hit
CCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAA55500.14589272547326418No Hit
GTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCAAGACGAGA54420.14305373189648715No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT53800.1414239392875966No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG52990.13929469410501383No Hit
CAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC51470.1352990735154758No Hit
CAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTG51080.13427388139052854No Hit
AATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCAAGACGAGAAGAC50830.13361670695145977No Hit
CTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCTAAAC50160.1318554794547555No Hit
GGCGGGCATAACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTAT50020.13148746176887702No Hit
CTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT49580.130330834756116No Hit
GCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAAC49040.12891133796772747No Hit
CCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAA48100.12644036207682896No Hit
TGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAACT46950.12341735965711266No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT45220.11886971253875686No Hit
CACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGG44330.11653017153567208No Hit
AATAACTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCT43880.11534725754534833No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT43550.11447978728577755No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG43130.11337573422814204No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG42530.11179851557437702No Hit
CCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTAC42520.11177222859681428No Hit
CTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGAC42430.11153564579874953No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA41680.10956412248154326No Hit
CAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCC40660.10688285077014273No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA40570.10664626797207796No Hit
CGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCC40510.10648854610670147No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG40190.10564736282469346No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA40110.10543706700419145No Hit
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC39770.10454330976705795No Hit
GGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAA39490.10380727439530094No Hit
CCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCT39320.10336039577673418No Hit
GCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAA38860.10215119480884768No Hit
CTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTC38780.10194089898834567No Hit
TCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTT38550.1013362985044024No Hit
GCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAAC38480.10115228966146317No Hit
CTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAG38170.1003373933570179No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)