FastQCFastQC Report
Wed 8 May 2024
EGAF00007858596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007858596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2747782
Total Bases277.5 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG859413.1276498645089026TruSeq Adapter, Index 3 (97% over 35bp)
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT59300.21581042455333066No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA52110.18964386548860135No Hit
GGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGT49430.1798905444463935No Hit
GTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTG47820.17403127322327608No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC46440.16900904074631828No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG43400.15794557210142582No Hit
TGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTT38790.14116840418926974No Hit
TCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGC37810.13760189127085046No Hit
CTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT35830.13039607945608495No Hit
TCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG35820.13035968646712148No Hit
GGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCG35160.127957749195533No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT33990.1236997694868079No Hit
GGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATC31080.11310940969844042No Hit
GCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAAT30860.11230876394124426No Hit
CGGCTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTGGCTGGCACGAA29440.10714095950843261No Hit
GTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGT29280.10655867168501722No Hit
TTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCATCGGGATGT28270.10288297979970755No Hit
TTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCCAGGTTTCAATTTC27740.10095415138464406No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)