FastQCFastQC Report
Mon 22 May 2023
EGAF00007858622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007858622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2644440
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG702802.6576515254647486TruSeq Adapter, Index 3 (97% over 35bp)
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT65550.24787856786313928No Hit
GGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGT53660.2029163074223654No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA52610.19894571251380255No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC47270.17875240126453995No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG44220.16721876843490494No Hit
TCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGC39720.15020193311249264No Hit
CTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT39450.1491809229931479No Hit
TGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTT38580.14589100149748152No Hit
TCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG36020.13621031295850916No Hit
GGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCG35430.1339792167717929No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT31580.11942036877372902No Hit
GGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATC31410.11877751055043789No Hit
GCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAAT30250.11439094855621607No Hit
TTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCATCGGGATGT30150.11401279666016245No Hit
GTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTG29260.11064724478528536No Hit
CGGCTTCTATTGACTTGGGTTAATCGTGTGACCGCGGTGGCTGGCACGAA28950.10947497390751917No Hit
GGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT28910.10932371314909774No Hit
GTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGT28800.10890774606343875No Hit
TGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTA27670.10463462963803301No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)