FastQCFastQC Report
Wed 8 May 2024
EGAF00007858639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007858639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3421489
Total Bases345.5 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC392031.1457876965262785Illumina Single End PCR Primer 1 (96% over 32bp)
TAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAATG176590.5161203207141686No Hit
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACC168200.49159883313960673No Hit
CTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTC99720.2914520549386539No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT73730.21549097483580976No Hit
GGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGG72470.21180836764344413No Hit
TGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC71070.20771658187414893No Hit
CCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAAT64200.1876376045633933No Hit
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGC59860.17495306867857827No Hit
CGACCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCAC59730.17457311714285798No Hit
GTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGAT58790.17182577526918835No Hit
CTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGT55710.16282384657673896No Hit
GCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC51590.15078230559852743No Hit
GAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG50860.14864873159025208No Hit
TCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAAGT50690.1481518718896948No Hit
CCAAATAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAG49960.14601829788141946No Hit
CCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATA47140.1377762722604106No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46560.13608110387027403No Hit
TCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCG45700.1335675783262784No Hit
CGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCG43560.12731299150749864No Hit
GTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAAC43330.12664076955968584No Hit
CGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTAAACTCCAT42640.1246241037162475No Hit
GGCACGAGACCGATAGTCAACAAGTACCGTAAGGGAAAGTTGAAAAGAAC41940.12257821083159993No Hit
TGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGC41400.12099995060630035No Hit
GGGTCTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGT41220.12047386386453383No Hit
GCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC41210.12044463682332458No Hit
GGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAA40360.11796033832053822No Hit
AACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATATC40270.11769729494965496No Hit
TCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTT39640.11585599135347213No Hit
CCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTC37570.10980599382315712No Hit
TTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCG37320.10907531779292583No Hit
CTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAA36990.10811082543302054No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT36630.1070586519494875No Hit
CTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGG36590.10694174378465048No Hit
GCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAAT36430.10647411112530247No Hit
GACCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCACC35910.10495430498242139No Hit
TGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCC35780.10457435344670113No Hit
TCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCGT34600.10112556258400948No Hit
AAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCA34260.10013184318289493No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)