FastQCFastQC Report
Wed 8 May 2024
EGAF00007858943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007858943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4031703
Total Bases407.2 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCA90980.22566146365444079No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG88990.22072558420101876No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC66770.1656123975401958No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC64860.16087494540148417Illumina Single End Sequencing Primer (100% over 31bp)
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG44100.10938305723412663No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA40470.1003794178291407No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)