FastQCFastQC Report
Wed 8 May 2024
EGAF00007859077

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007859077
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4062345
Total Bases410.2 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATCTC135450.3334281061800512Illumina Single End Sequencing Primer (100% over 31bp)
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC125920.30996874957690695No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG86010.21172500120004578No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG76470.18824102827307873No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT59240.14582710232636567No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT57400.14129769874296744No Hit
CCTCGATGTTGGATCAGGACATCCCAATGGTGCAGCCGCTATTAAAGGTT54170.13334662614819767No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA50000.12308161911408312No Hit
ACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAA48010.1181829706733426No Hit
CCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAA43470.10700715965778387No Hit
GGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATA41890.10311778049377884No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT41760.10279776828408223No Hit
CTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAG40970.10085307870207971No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)