FastQCFastQC Report
Mon 22 May 2023
EGAF00007859319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007859319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3980849
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC396520.996068929014891Illumina Single End PCR Primer 1 (96% over 33bp)
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGC139030.3492471078405637No Hit
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCTAAACCATTTACC116230.2919728932195117No Hit
GTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGAT80790.20294665786117486No Hit
CGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCG78380.19689267289465134No Hit
GGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGG67260.16895893313210322No Hit
GCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAAT63970.1606943644433637No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT62040.15584615241623082No Hit
TGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC60840.1528317200677544No Hit
CTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTC56390.14165320010882101No Hit
TTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCG55210.13868900829948586No Hit
GCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC51570.12954523017577407No Hit
CCCACTCCACCTTACTACCAGACAACCTTAGCTAAACCATTTACCCAAAT50360.12650567755772701No Hit
TCCACCTTACTACCAGACAACCTTAGCTAAACCATTTACCCAAATAAAGT49590.12457141680078797No Hit
GCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC43490.10924805236269951No Hit
TGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCA42500.10676114567520648No Hit
CCACTCCACCTTACTACCAGACAACCTTAGCTAAACCATTTACCCAAATA39920.10028011612598217No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)