FastQCFastQC Report
Mon 22 May 2023
EGAF00007859327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007859327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4124878
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC298910.7246517351543488Illumina Single End PCR Primer 1 (96% over 33bp)
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGC135950.3295855053167633No Hit
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCTAAACCATTTACC118920.2882994357651305No Hit
CGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCG79320.1922965964084271No Hit
GTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGAT78330.18989652542450952No Hit
GGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGG71510.17336270309085505No Hit
TGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC66520.16126537560626036No Hit
GCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAAT65770.1594471400123834No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT62660.15190752308310695No Hit
CTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTC61570.14926502068667244No Hit
TTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCG58630.14213753715867475No Hit
GCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC58150.14097386637859352No Hit
CCCACTCCACCTTACTACCAGACAACCTTAGCTAAACCATTTACCCAAAT48400.11733680365819303No Hit
TCCACCTTACTACCAGACAACCTTAGCTAAACCATTTACCCAAATAAAGT48160.1167549682681524No Hit
GCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC47640.1154943249230644No Hit
TGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCA43530.10553039386861865No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)