FastQCFastQC Report
Wed 8 May 2024
EGAF00007859335

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007859335
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3738969
Total Bases377.6 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC302500.8090465580217434Illumina Single End PCR Primer 1 (96% over 33bp)
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGC126880.3393448835761944No Hit
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCTAAACCATTTACC104660.2799167364051427No Hit
CGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCG76190.20377275125843514No Hit
GTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGAT75800.2027296829687542No Hit
GGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGG68240.1825102053534009No Hit
TGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC63740.17047480201092868No Hit
GCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAAT62390.166864181008187No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT57890.15482877766571482No Hit
CTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTC54520.14581559782924114No Hit
GCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC54040.1445318214727108No Hit
TTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCG53610.14338177181998568No Hit
CCCACTCCACCTTACTACCAGACAACCTTAGCTAAACCATTTACCCAAAT45340.12126337501059785No Hit
GCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC44460.1189097850236255No Hit
TCCACCTTACTACCAGACAACCTTAGCTAAACCATTTACCCAAATAAAGT43140.11537940004316698No Hit
TGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCA42220.11291882869315042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)