FastQCFastQC Report
Mon 22 May 2023
EGAF00007859337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007859337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3598641
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC298170.8285627824503751Illumina Single End PCR Primer 1 (96% over 33bp)
GGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGC125350.34832593748584534No Hit
CCAAACCCACTCCACCTTACTACCAGACAACCTTAGCTAAACCATTTACC102640.28521878120101446No Hit
CGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCG73920.2054108759390003No Hit
GTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGAT72700.20202070726143564No Hit
GGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGG66400.18451409851663447No Hit
GCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAAT60840.16906382159265124No Hit
TGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATC59610.16564586464723766No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT55710.15480844018617027No Hit
CTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTC53140.14766685534900537No Hit
GCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC52800.14672205424214307No Hit
TTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCG51850.14408216879649846No Hit
CCCACTCCACCTTACTACCAGACAACCTTAGCTAAACCATTTACCCAAAT43430.12068444726773248No Hit
TCCACCTTACTACCAGACAACCTTAGCTAAACCATTTACCCAAATAAAGT43380.12054550592848799No Hit
GCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTC43070.11968406962517238No Hit
TGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCA40730.11318161494853196No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)