FastQCFastQC Report
Wed 8 May 2024
EGAF00007860182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007860182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4990488
Total Bases374.2 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG203720.40821659124318105No Hit
CTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGT143470.2874869151073001No Hit
GGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATC128500.2574898486881443No Hit
CTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGGC122810.24608815811199225No Hit
GATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATT122040.24454522283191543No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA118960.23837348171160816No Hit
GTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGT118430.2373114613240228No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG118220.2368906607930928No Hit
GGGATTTAGAGGGTTCTGTGGGTAAATTTAAAGTTGAACTAAGATTCTAT116430.23330383721992717No Hit
GCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCA115060.2305586147086217No Hit
GTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTG115000.23043838598549882No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG111800.2240261874189458No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG110880.2221826803310618No Hit
TATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGA110570.221561498594927No Hit
GCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGT109730.21987829647120682No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA108150.21671227342897129No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG99330.19903865112990954No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG97290.194950874543732No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG93860.188077799205208No Hit
CGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAA92880.18611406339420114No Hit
GTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCA88480.17729729036519074No Hit
GGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT87900.176135079375003No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG85850.17202726466830498No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA84240.168801127264508No Hit
ACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAAGTAGG82800.16591563790955915No Hit
CGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGG79670.15964370618664947No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC77810.15591661576984053No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT77600.15549581523891048No Hit
CTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAA77330.1549547859848576No Hit
GCACCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTT76860.1540129943203951No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTA76050.1523899065582364No Hit
GGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGG75590.1514681530142944No Hit
GTAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCACC74260.14880308298507078No Hit
GATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTT74010.14830212997205883No Hit
ATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTGAA73700.147680948235924No Hit
GTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGTGAAG68960.1381828791092174No Hit
GCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATT67880.13601876209300573No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTT66730.13371437823315074No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCA62960.12616000679693049No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC62340.12491764332466083No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA62260.12475733836049702No Hit
GTGAGTTTTAGCTTTATTGGGGAGGGGGTGATCTAAAACACTCTTTACGC61190.12261325946480585No Hit
CACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAG60120.12046918056911468No Hit
CTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT58580.11738331000896104No Hit
GGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCCGGTCTGAACTCA58350.11692243323699004No Hit
TGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTA58140.11650163270606No Hit
GTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTT57540.11529934547483131No Hit
GCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATT57400.11501881178754463No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC57160.11453789689505314No Hit
TGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAG56730.11367625771267259No Hit
GGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTA56690.11359610523059067No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT56450.11311519033809919No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT55960.11213332243259577No Hit
GCTGCTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAG55260.11073065399616229No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA54980.11016958662158892No Hit
AAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGT53240.1066829536510257No Hit
GTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATT52630.10546062829927655No Hit
CTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGAC52360.10491959904522362No Hit
CACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTG52360.10491959904522362No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCT51150.10249498646224577No Hit
TGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGC51080.10235471961860243No Hit
GTAGGTTTGTCGCCTCTACCTATAAATCTTCCCACTATTTTGCTACATAG51000.10219441465443861No Hit
GTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGG50990.10217437653391812No Hit
GTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTATG50230.1006514793743618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)