FastQCFastQC Report
Fri 10 May 2024
EGAF00007861458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007861458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2822084
Total Bases211.6 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG455601.614409776604807No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG394551.3980802839320163No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA324791.1508870749417806No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA257940.9140053945949164No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC234070.8294225118742036No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT233040.8257727268217389No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT174570.6185854141832773No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT158240.5607203754388601No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG152720.5411603623421557No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC144190.5109344725387338No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT130430.46217617902231123No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC122800.43513942178900417No Hit
GGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTA108980.38616851943457386No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC102330.36260437322205863No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT97100.34407196950905783No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT87670.3106569471355211No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC85130.30165650632653035No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG84430.2991760698831077No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT83860.2971562859220349No Hit
GCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGG82830.29350650086957014No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC79540.28184844958548366No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC79140.2804310573320993No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT78280.27738366398732284No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA70820.25094929846170416No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG70410.2494964714019852No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC66720.23642102786451433No Hit
GCGGAGGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTACCT66510.23567689693148752No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG66020.23394059142109164No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT64560.22876710969623867No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC60460.2142388390990488No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA60130.21306949049000665No Hit
CGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCC56230.19924991601950898No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG55770.19761991492811695No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA55600.1970175232204286No Hit
GGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTT53630.19003686637251052No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC53580.18985969234083747No Hit
GTCTGTTCCAAGCTCCGGCAAAGGAGGCATCCGCCGGGCCCCTCCCCGAA50120.1775992493490626No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA47070.16679163341700673No Hit
GTGTGGTTGGTGCGCGGACACGCACTGCCTGCGTAACTAGAGGGAGCTGA47060.16675619861067212No Hit
GTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA44900.15910228044239647No Hit
AGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGA44570.15793293183335436No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT44260.15683445283698147No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC41300.14634575016193707No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA41290.14631031535560246No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG38200.13536096019820815No Hit
TATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGC37510.13291595856112007No Hit
GGAGAGTAGTCTGAATTGGGTTATGAGGTCCCCTGCGGGGTACCTCACCT37280.13210095801542407No Hit
CTCGTATACCCTTGACCGAAGACCGGTCCTCCTCTATCGGGGATGGTCGT36330.12873465141363616No Hit
CGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCA35990.12752986799825944No Hit
TCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC35610.12618334535754427No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG32970.11682855648520737No Hit
GGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATACCCTTGACCGAAGAC31850.11285985817573113No Hit
ACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTG30800.10913920351059714No Hit
GTGGTTGGTGCGCGGACACGCACTGCCTGCGTAACTAGAGGGAGCTGACG29530.10463898310610173No Hit
CTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG29360.10403659139841337No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)