FastQCFastQC Report
Mon 22 May 2023
EGAF00007861717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007861717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4107736
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG228260.5556832279386991No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA195900.4769050396617504No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG186020.452852861040729No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT153320.3732469662120448No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA148150.3606609577635953No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT147420.3588838231083984No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG136650.33266500086665746No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC131810.32088235465959836No Hit
CATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGG128780.31350602862501387No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT128160.31199668138361375No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA125630.3058375708662874No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCA124130.3021859243145129No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT124060.30201551414209676No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT123290.30014100224551915No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC121060.2947122210385478No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG119650.29127967327987975No Hit
CGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGA115560.2813228503487079No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT102680.24996737862413748No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG102520.24957786965861486No Hit
CACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCA100420.24446556448613055No Hit
CTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCT100090.2436622022447402No Hit
CGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAA98930.2408382622447012No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAG97320.2369188282791299No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG96090.2339244781066748No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT94960.23117357103767136No Hit
GATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG93840.22844700827901304No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT93790.22832528672728725No Hit
GGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAA92370.22486839465827407No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT91420.22255568517548352No Hit
CTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG87400.21276927241672788No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT86140.20970188931323727No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC80650.19633686293374258No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAG80630.19628817431305226No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCA76970.18737815672672248No Hit
TGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGA76830.18703733638189016No Hit
CGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTA76800.18696430345085469No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC75530.18387257603701893No Hit
CGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTA74020.18019658517489928No Hit
GTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCC72860.17737264517486032No Hit
CCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGC72750.17710485776106352No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG69430.16902254672646927No Hit
GGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA68620.16705065758851104No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT65190.15870055914012No Hit
GGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT63310.1541238287952293No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC61990.15091037982966773Illumina Single End PCR Primer 1 (96% over 31bp)
GTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGC61040.1485976703468772No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC59710.14535987707097048No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG59510.1448729908640672No Hit
GGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT58710.14292544603645416No Hit
CTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGG57800.14071011379504428No Hit
GCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACC57550.1401015060364152No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG57540.14007716172607004No Hit
TGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCG57510.14000412879503454No Hit
GATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAG56020.1363768265536052No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT55940.13618207207084387No Hit
CGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT53530.13031509327765953No Hit
ACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAG52060.1267364796569205No Hit
CTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTG50690.12340130913963313No Hit
ATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGT49450.12038261465683288No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC49030.11936015362233601No Hit
GTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAG48500.11806990517404235No Hit
CAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCT47140.11475907896710014No Hit
GGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACG47040.11451563586364849No Hit
CCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGG46170.11239768086361927No Hit
GCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGG45350.11040144741531588No Hit
TGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG44950.10942767500150936No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT43870.1067984894842317No Hit
CCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTA43750.10650635776008974No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG43600.1061411931049123No Hit
CTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTG42810.10421799258764439No Hit
GTGTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGA42320.10302512138073139No Hit
TGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTA41810.10178356155312807No Hit
CAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCT41700.10151577413933124No Hit
GTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAG41680.10146708551864092No Hit
GGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAA41190.10027421431172791No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)