FastQCFastQC Report
Thu 9 May 2024
EGAF00007862365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007862365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4203581
Total Bases424.5 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC300600.7151045739335106Illumina Single End Sequencing Primer (100% over 31bp)
CCCAAACCCACTCCACCTTACTACCAGATAACCTTAGCCAAACCATTTAC155540.37001784906725954No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG138480.3294334045186711No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG119480.28423384728401807No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT106450.25323646671730604No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG101310.2410087970232999No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA98760.23494254065759648No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA98280.23380065710640524No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG93910.22340475894243503No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT91920.2186707000531214No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA87760.20877437594279735No Hit
CTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGT85080.20239885944864627No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT81490.19385852205536183No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG76800.1827013681905975No Hit
TTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTT71390.1698313889990463No Hit
CATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGG69920.16633437062352313No Hit
CCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTT68500.16295629845124907No Hit
CCCCAAACCCACTCCACCTTACTACCAGATAACCTTAGCCAAACCATTTA66820.15895970602207973No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT66090.15722309145464308No Hit
CGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGA65950.15689004208554563No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCA64220.1527745034531272No Hit
GGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATA63590.15127578129218874No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT63560.15120441357023928No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG63480.15101409964504073No Hit
ACCCACTCCACCTTACTACCAGATAACCTTAGCCAAACCATTTACCCAGA61870.14718403190042015No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT61610.1465655116435249No Hit
GAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATACCTTC61130.14542362809233364No Hit
CACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCA61050.1452333141671351No Hit
CCTGGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG59660.14192660971681048No Hit
CCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAA59080.14054683375912108No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG58460.13907190083883242No Hit
CTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGAC58270.13861990526648588No Hit
CTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAG57720.13731149703074594No Hit
CTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCT56930.13543214701941036No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT56810.13514667613161255No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC56770.13505151916901328No Hit
GTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGA54790.13034124952034945No Hit
GCTCTTTAGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAAC53810.12800990393666734No Hit
CAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTG53560.1274151729204219No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC53350.12691559886677573No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT52480.12484593493024161No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT50810.12087313174172212No Hit
CTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCTAAAC50560.12027840072547669No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC50120.11923167413688471No Hit
GTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCC49650.11811357982634331No Hit
CTTGACCAACGGAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCT49650.11811357982634331No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAG49580.11794705514179457No Hit
CAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC49540.1178518981791953No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT49260.11718579944100044No Hit
AATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGAAGAC48500.1153778171516143No Hit
CTTAGCCAAACCATTTACCCAGATAAAGTATAGGCGATAGAAATTGAAAC48470.11530644942966484No Hit
CGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAA48190.11464035069146997No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAG47690.1134508886589791No Hit
GATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG47550.11311783928988166No Hit
TGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAACT46990.11178564181349188No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT45590.10845514812251746No Hit
CACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGG45460.10814588799406982No Hit
CCTTAGCCAAACCATTTACCCAGATAAAGTATAGGCGATAGAAATTGAAA45360.10790799558757164No Hit
CAGCTCTTTAGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTA44290.10536254683804118No Hit
GGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAA44120.1049581297469943No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT43990.10464886961854666No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA43810.10422066328684995No Hit
CTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGAC43130.10260299492266237No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG42680.10153247909342059No Hit
GGCGGGCATGACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTAT42460.1010091157991246No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)