FastQCFastQC Report
Wed 8 May 2024
EGAF00007862682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007862682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4103019
Total Bases414.4 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG526051.282104713626722TruSeq Adapter, Index 1 (97% over 35bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT238410.5810599463468241No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT172930.42147014186383247No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA141220.34418558627196216No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT140040.3413096551587989No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA115410.2812806862459082No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG112610.27445644292653776No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC110670.2697282171981168No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA108970.2655849266113562No Hit
CTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCA108470.26436631173289715No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG105460.2570302501645739No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT104370.2543736697295333No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA104270.2541299467538415No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA102680.25025475144034187No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG99940.24357674190638648No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT95230.23209738975130262No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT93040.22675985658365216No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC72720.1772353479230781No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT67390.16424491331870508No Hit
CTCCGGGCGGACTGCGCGGACCCCACCCGTTTACCTCTTAACGGTTTCAC63410.15454473888617137No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC61350.14952404558692028No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA60250.14684309285431046No Hit
CCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCC60240.14681872055674125No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT58920.1436015772776095No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG57580.14033568940333935No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT57390.13987261574952492No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA57180.13936079750057215No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC56800.1384346501929433No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC53720.13092798254163582No Hit
CCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAAT52690.12841763589201025No Hit
ATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGA52660.1283445189993027No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA52510.127978934535765No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA51200.1247861635542024No Hit
CCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTC49480.1205941283723034No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG49450.12052101147959587No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC48280.1176694526640018No Hit
CTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG47270.1152078506095146No Hit
CCCCACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTT46980.11450105398000839No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT45940.11196633503281364No Hit
CCCCGCTTCGCGCCCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTT45900.11186884584253691No Hit
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC44690.10891979783666612No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC44620.10874919175368186No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTA44600.10870044715854352No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC44500.10845672418285171No Hit
CTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCAT44370.10813988431445234No Hit
GTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT43030.10487399644018222No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA42210.10287546803950945No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT42040.10246113898083338No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC41790.10185183154160388No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG41430.1009744288291134No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)