FastQCFastQC Report
Wed 8 May 2024
EGAF00007863407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007863407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5905645
Total Bases442.9 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG257150.43543084624964756No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC144640.24491820961131255No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG100940.17092121182360268No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA99280.16811034188475604No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC94170.15945760369951123No Hit
TGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCG81620.13820674964377305No Hit
CTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGG68010.1151610027355183No Hit
GCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGAC61270.10374819346574338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)