FastQCFastQC Report
Mon 22 May 2023
EGAF00007864189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007864189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3313183
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG119520.3607407136883172No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC90270.27245702999200466Illumina Single End PCR Primer 1 (96% over 31bp)
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA43360.13087112906229448No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA41740.12598157119603715No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC40360.12181639227292908No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC37050.1118259993486626No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG36310.1095924976072858No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT36270.1094717677834276No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAA35190.10621206253925607No Hit
GGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCT34840.10515567658049675No Hit
GGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTC34000.10262035027947444No Hit
ACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGA33540.10123195730510509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)