FastQCFastQC Report
Wed 31 May 2023
EGAF00007864193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007864193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3605458
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG130470.3618680345187768No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC53430.14819199114231812Illumina Single End PCR Primer 1 (96% over 31bp)
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAA50580.14028730885230115No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA44160.12248097190426294No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAA43740.12131607135626042No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC43480.1205949424455922No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT41960.1163791118909165No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC41170.11418798943157846No Hit
GGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTA38830.10769782923556452No Hit
GGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCT38100.10567312114022685No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG38000.10539576386689291No Hit
GGGCAACGGAGGCCATCGCCCGTCCCTTCGGAACGGCGCTCGCCCATCTC37230.10326011286222166No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA37040.1027331340428872No Hit
ACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGA36510.10126314049421738No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)