FastQCFastQC Report
Wed 8 May 2024
EGAF00007864302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007864302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4223649
Total Bases426.5 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT114010.26993246834668316No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT110990.26278225297604035No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG89680.2123282498143193No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG85170.20165027917802827TruSeq Adapter, Index 2 (97% over 36bp)
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC78180.185100608502269No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT73790.17470675238401676No Hit
GGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGG66260.15687856637708295No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG64610.15297199175404963No Hit
CTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTT62970.1490890933408529No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTA61910.14657941509817696No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA61500.14560869049487776No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA61440.1454666332358584No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA57340.1357593872028665No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT56960.13485969122907704No Hit
GTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATT55730.13194751741917948No Hit
GTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTG51690.12238232864520701No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG51240.12131689920256157No Hit
GCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCA50690.12001470766155047No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC49790.11788384877625957No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTA49510.11722091490083575No Hit
GGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTA47990.11362213100567779No Hit
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT45630.10803454548424833No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT45470.10765572612686329No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT44810.10609309627764997No Hit
GGGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCC43840.1037965039235031No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCT42710.10112109221197121No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTT42690.10107373979229808No Hit
GCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGT42560.10076594906442272No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG42330.10022139623818173No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)