FastQCFastQC Report
Wed 8 May 2024
EGAF00007864832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007864832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4527295
Total Bases457.2 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG542201.197624630159952TruSeq Adapter, Index 6 (97% over 35bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT243130.5370314945237719No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT189760.4191465323112366No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC162500.3589339771320402No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT127020.2805648847711492No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT126140.2786211192334495No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG107720.2379345724102361No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA107370.2371614838441056No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA107070.23649883650170797No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT102800.22706715599491528No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG95490.21092064908516012No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG94840.20948491317663198No Hit
CTGCTGTTTCCCGTGGGGGTGTGGCTAGGCTAAGCGTTTTGAGCTGCATT92590.20451505810864987No Hit
CGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTA86300.19062155216304658No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA84930.18759546263276417No Hit
GTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATT78920.17432042754006533No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA78110.17253127971559176No Hit
GTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTG77380.1709188378490909No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG74490.16453533511732724No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG73530.1624148636216549No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGT72220.15952130355985197No Hit
CTCTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGAC71840.15868195025948167No Hit
GTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTT70930.15667191998754224No Hit
CTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATG68820.15201130034601235No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCT67370.1488085048577572No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC66900.14777035735466765No Hit
GTGAGTTTTAGCTTTATTGGGGAGGGGGTGATCTAAAACACTCTTTACGC65760.1452522974535567No Hit
GGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGG65410.14447920888742616No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTT64900.14335270840535022No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG64750.1430213847341514No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT63390.14001738344861558No Hit
GTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAGCA63260.13973023626690995No Hit
CTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCTATTGCGCC62710.13851538280584766No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT62180.13734470583427855No Hit
GTCTGGTTTCGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTA61390.13559973449929816No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG60880.1344732340172222No Hit
GGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT60280.133147939332427No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC60150.13286079215072136No Hit
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT57060.12603552452402594No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC56870.12561584787384078No Hit
GGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTA56550.12490902404194998No Hit
CCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATA55900.12347328813342184No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGA55580.12276646430153106No Hit
GTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAG54520.12042511035839283No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA53400.11795122694677505No Hit
GGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCT50990.11262795996284758No Hit
GGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTG50970.1125837834733544No Hit
CGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGG47390.10467619185407621No Hit
CTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACT47260.10438904467237058No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA46950.10370430908522639No Hit
CCAGCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTC46010.1016280140790472No Hit
CTGGTTTCGGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGT45310.10008183694678612No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)