FastQCFastQC Report
Wed 8 May 2024
EGAF00007865030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007865030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3473443
Total Bases350.8 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC519891.4967569641994989No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC431331.2417938051668043No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG347741.0011392154700682No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC242470.6980681704003779No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG222120.6394807687933846TruSeq Adapter, Index 19 (97% over 37bp)
CGGCCTTCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACT185340.5335915977317032No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA169680.48850664887836076No Hit
GCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA147860.42568713521425283No Hit
CGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCC136900.39413342899250114No Hit
CGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTC107900.3106427829677931No Hit
CCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGT106350.3061803518871621No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC106280.3059788227415852No Hit
GCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAAC101630.29259152949969236No Hit
CCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAAC99400.28617138671917175No Hit
GGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCAT98290.28297571026788115No Hit
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT95750.27566308127123434No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT93210.2683504522745875No Hit
CGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTC90400.2602604965735727No Hit
CGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAACT86980.25041435831824504No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG83760.24114401762170848No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG83060.23912872616593966No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC82400.2372285942219291No Hit
TCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC80910.23293890240893544No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG78920.22720971669896411No Hit
GAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAG78460.2258853823137446No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA77320.22260333622863537No Hit
ATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCT77010.2217108500125092No Hit
TCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGC76330.21975313831261947No Hit
GCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGG76030.21888944197443286No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG74820.21540586674374676No Hit
GCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA73960.21292993724094508No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA73840.21258445870567044No Hit
GGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGT71880.20694164262951772No Hit
CTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCAC69740.2007806087504531No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC65410.18831459160262598No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA64530.1857810823439452No Hit
GAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAACTC64150.1846870669822421No Hit
TGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGG63830.18376579088817635No Hit
TCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCC59850.1723074194682337No Hit
CTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT58220.16761466936408628No Hit
TAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCG56710.16326739779521357No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG54730.15756700196318177No Hit
TTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTT54100.15575323965298984No Hit
TACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGG53450.15388189758691878No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT52420.15091654015914469No Hit
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT52210.15031195272241404No Hit
TGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTT51510.14829666126664523No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA50960.14671321797996975No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC50820.14631015968881597No Hit
AGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGA50460.145273724082992No Hit
GGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGC50390.14507219493741513No Hit
TCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGC50380.14504340505947555No Hit
GGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTA50350.1449570354256569No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG50110.1442660783551076No Hit
GGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGC49090.14132951080527303No Hit
GGCCTTCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACTC48350.139199059837746No Hit
CGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCA47830.13770198618488916No Hit
TATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGC47590.13701102911433985No Hit
CGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC46440.1337001931512911No Hit
GTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCAC45990.13240464864401114No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC45620.13133942316024763No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT44540.12823011634277576No Hit
TCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG43560.1254087083046994No Hit
TGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG40900.1177506007727779No Hit
TGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTA39930.11495798261264112No Hit
CCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCC39640.11412307615239403No Hit
TTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCATCGGGATGT39200.11285632152305365No Hit
TCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCC39030.11236689359808122No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT38170.10989096409527953No Hit
TATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACT37920.10917121714679066No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC37550.10810599166302716No Hit
GTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCG37260.10727108520278007No Hit
GGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT36680.10560127228228591No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC36200.1042193581411873No Hit
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC36130.10401782899561042No Hit
ACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCAG35870.10326929216918199No Hit
TCACCTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCA35490.10217527680747893No Hit
GCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTA35380.10185858815014383No Hit
GCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAAT35290.10159947924868783No Hit
GGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCA35200.10134037034723184No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)