FastQCFastQC Report
Mon 22 May 2023
EGAF00007865249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007865249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4186388
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC218260.5213563577957896Illumina Single End PCR Primer 1 (96% over 31bp)
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC106920.25539916510366456No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG56170.13417294335833183No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT54700.1306615631422601No Hit
CTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGT51930.12404488069428825No Hit
CCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTA48950.1169265725011633No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGT47890.1143945568351524No Hit
CTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAAC42540.10161504380387103No Hit
CCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAA42400.10128062664043562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)