FastQCFastQC Report
Wed 8 May 2024
EGAF00007865293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007865293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3988954
Total Bases402.8 Mbp
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC227030.5691466986082065Illumina Single End PCR Primer 1 (96% over 32bp)
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC82420.20662058273923442No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCG40190.10075323004476863No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)