FastQCFastQC Report
Mon 22 May 2023
EGAF00007866319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007866319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3570307
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG130700.36607496218112334No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA110550.30963723847837177No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG95750.2681842205726286No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC93130.26084591605147683Illumina Single End PCR Primer 1 (96% over 32bp)
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA92380.2587452563603074No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG89030.24936230973975065No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAG81340.2278235457062936No Hit
CCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCA79100.2215495754286676No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG75460.21135437372752536No Hit
GCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAG74550.20880557330223987No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC71970.20157930396461707No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCA67090.18791101157407472No Hit
GGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAA66240.18553026392408273No Hit
CATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGG65540.1835696482123246No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT57150.16007026846710942No Hit
CGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAA56490.15822168793888033No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC52740.14771838948303323No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT51690.1447774659153961No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC51170.14332100852951862No Hit
CCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCA50510.14147242800128954No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT49910.139791900248354No Hit
CGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGA49720.13925973312659107No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG49410.13839146045424106No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT46220.12945665456780048No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG46210.1294286457719182No Hit
CACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCA45970.128756434670744No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC45440.12727196848898428No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG44860.12564745832781327No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT44390.1243310449213471No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT44190.12377086900370192No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT43110.12074591904841796No Hit
TGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGA42100.11791703066430984No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT41200.11539623903490653No Hit
CGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGG40340.11298748258903227No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT40200.11259535944668064No Hit
GATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG39910.11178310436609512No Hit
GCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGG39160.10968244467492572No Hit
GCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACC37360.10464086141611913No Hit
GATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAG36390.10192400821554001No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC35720.10004741889142867No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)