FastQCFastQC Report
Wed 31 May 2023
EGAF00007866872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007866872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4218485
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC307210.7282472261961344No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG245290.5814646727438879TruSeq Adapter, Index 8 (97% over 35bp)
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG221450.5249514932493538No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC205120.4862409135033075No Hit
CCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATT135480.3211579512550122No Hit
GGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGT126530.29994180375182083No Hit
TGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTG119740.28384597788068466No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGG114820.27218302305211467No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC94520.22406148178789306No Hit
GGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACA88440.20964872460136752No Hit
CGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCC86800.2057610729918442No Hit
GGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTAC86730.20559513664265724No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC82720.1960893543535179No Hit
CTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTT77530.18378635932094106No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT73360.17390129394794576No Hit
GCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCA72920.17285826546734195No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA70960.16821204769010675No Hit
TGGTTTCGGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTT69680.16517778301925928No Hit
TCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGC66740.1582084563534065No Hit
TCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTC64250.15230586336089852No Hit
CTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGG63670.15093096218192076No Hit
CCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGT63540.1506227946762878No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA58840.13948135408801976No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG58260.138106452909042No Hit
TCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG58100.13772716982518607No Hit
CGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTC54690.12964369910050647No Hit
GTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTG53900.1277709888739678No Hit
TTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCATTGGGATGT52540.12454708266119235No Hit
GCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGG51900.12302995032576862No Hit
TGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTA50060.11866819486142538No Hit
CCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAAC49350.11698512617681467No Hit
GGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATC48080.11397456669870819No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC46570.11039508259481781No Hit
GGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCATT46080.10923352815050902No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT45300.10738452311671133No Hit
ATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTT45150.10702894522559639No Hit
GGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGG45090.10688671406915043No Hit
GTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGG44390.10522735057728072No Hit
GTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGTTCGCTTTGACTGGT44150.1046584259514968No Hit
GGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCAT43670.103520576699929No Hit
GCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAAT42670.10115005742582941No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG42650.10110264704034741No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)